Modelo experimental en ratas para el estudio de osteomielitis relacionada con biomaterialesinfluencia de agentes físico-químicos sobre implantes de Ti6Al4V
- Constantino Cabrera, Juan Antonio
- Manuel Quiles Galindo Director
- Ciro Pérez Giraldo Director
Defence university: Universidad de Extremadura
Fecha de defensa: 10 June 2014
- Antonio C. Gómez García Chair
- Rafael Lorente Moreno Secretary
- Tomás Ángel Sanz Gómez Committee member
- Enrique García Sánchez Committee member
- Ignacio Trujillano Martín Committee member
Type: Thesis
Abstract
In the present thesis a model of osteomyelitis in Wistar male rats is developed, in order to study in vivo the effectiveness of new physica- chemical strategies, such as ultraviolet radiation, and farnesol, in preventing colonization by� S. aureus� Tiv(6)Alv(4)V alloy. 20 Rats which were operated by the surgical implantation of a wire of Tiv(6)Alv(4)V with different inocula of �S. aureus� in the femoral cavity were initially used in the creation of experimental osteomyelitis model. In our model, the minimum inoculum to generate an experimental osteomyelitis by �S. aureus� in rats associated with implants of Tiv(6)Alv(4)V was 1000 bacteria. To assess the effectiveness of UV radiation in the prevention of colonization of implants Tiv(6)Alv(4)V by �S. aureus� 56 rats were used, noting that the treatment of Tiv(6)Alv(4)V wires with ultraviolet radiation for 48 hours prior to implantation intramedullary in the femur of rats, decreased the rate of colonization by �S. aureus�, both in wires and femur. Last 18 rats were used to assess the effect of the farnesol in preventing colonization by �S. aureus� found that implantation of Tiv(6)Alv(4)V wires impregnated with a dissolution of farnesol (30 mM) in rats femurs, caused a decrease in the rate of colonization by �S. aureus� of these wires.