Incorporación de agentes anti-infecciosos en nanopartículas metálicas de oro y chitosan

  1. DÍAZ SÁNCHEZ, Salomé 1
  2. MARTÍNEZ LANAO, José 1
  3. COLINO GANDARILLAS, Clara Isabel 2
  1. 1 Departamento de Farmacia y Tecnología Farmacéutica. Facultad de Farmacia, Universidad de Salamanca. Campus Miguel de Unamuno
  2. 2 Universidad de Salamanca
    info

    Universidad de Salamanca

    Salamanca, España

    ROR https://ror.org/02f40zc51

Journal:
Farmajournal

ISSN: 2445-1355

Year of publication: 2018

Volume: 3

Issue: 2

Pages: 53-61

Type: Article

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Abstract

Resistance to antimicrobial agents is a serious health problem, causing great mortality in the population. This makes very interesting the search for alternatives for the treatment of these infections caused by multiresistant bacteria. One of them could be the use of gold metallic nanoparticles (NPs) together with an anti-infectious agent such as ciprofloxacin which is a broad-spectrum antibacterial drug. The objective of the study was to evaluate the behavior of gold-chitosan (Au-chitosan) NPs compared to gold-citrate (Au-citrate) NPs in the uptake of ciprofloxacin for different incubation conditions. The nanoparticles were synthesized and the anti-infectious agent incorporated at different study concentrations. By ultra-high performance liquid chromatography (UHPLC), the concentrations of ciprofloxacin incorporated into the nanoparticles were estimated. The percentage incorporation of ciprofloxacin was higher for Au-citrate nanoparticles compared to Au-chitosan nanoparticles. However,  Au-citrate disadvantage form large aggregates, difficulting its good management. Au-chitosan NPs are more stable and chitosan has antibacterial activities. Thus, gold and chitosan nanoparticles could be a good vehicle for the incorporation of anti-infective agents and may even have the potential to enhance their antimicrobial activity.

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