Expresión de los genes de mucinas MUC13, MUC15, MUC16 y MUC17 en el epitelio conjuntival normal humano in vivo

  1. RM Corrales
  2. DJ Galarreta
  3. JM Herreras
  4. M Calonge
  5. FJ Chaves
Revista:
Archivos de la Sociedad Española de Oftalmologia

ISSN: 0365-6691

Any de publicació: 2003

Volum: 78

Número: 7

Pàgines: 375-381

Tipus: Article

DOI: 10.4321/S0365-66912003000700006 DIALNET GOOGLE SCHOLAR lock_openAccés obert editor

Altres publicacions en: Archivos de la Sociedad Española de Oftalmologia

Resum

Purpose: The ocular surface epithelia express at least five mucin genes of the total of 17 human mucin genes that have been identified so far. This study was designed to determine the expression profile of mucin genes in conjunctival impression cytology (CIC) samples from healthy subjects. Methods: Two polyethersulfone filters were applied to the superior conjunctiva of both eyes from eight healthy donors. Polymerase chain reaction (PCR) was performed using isolated and retrotranscripted total RNA obtained from the CIC samples to study the expression of all known human mucin genes. Following amplification, PCR products were electrophoresed on 1.5% agarose gel and stained with ethidium bromide to confirm that only a single band was obtained when amplifying all cDNAs with the convenient primers. Results: Transcripts of the previously reported conjunctival mucin genes MUC1, MUC2, MUC4, MUC5AC, and MUC7 were detected in all samples. In addition, transcripts of MUC13, MUC15, MUC16 and MUC17 mucin genes also were detected. Amplified products by conventional PCR showed the expected amplicon size. Transcripts of MUC3A, MUC3B, MUC5B, MUC6, MUC8, MUC11, and MUC12 mucin genes were not detected. Conclusion: The expression of four additional mucin mRNA (MUC13, MUC15, MUC16, and MUC17) has been proved in human conjunctival epithelium from healthy donors for the first time. The function of these genes remains to be further elucidated.

Referències bibliogràfiques

  • Rohen, JW, Lütjen-Drecoll, E. (1992). The Dry Eye: A Comprehensive Guide. Springer-Verla. Heidelberg^eBerlin Berlin.
  • Prydal, JI, Campbell, FW. (1992). Study of precorneal tear film thickness and structure by interferometry and confocal microscopy. Invest Ophthalmol Vis Sci. 33. 1996-2005
  • Gipson, IK, Inatomi, T. (1997). Mucin genes expressed by the ocular surface epithelium. Prog Retin Eye Res. 16. 81-98
  • Gum, JR Jr, Hicks, JW, Toribara, NW, Siddiki, B, Kim, YS. (1994). Molecular cloning of human intestinal mucin (MUC2) cDNA: Identification of the amino terminus and overall sequence similarity to prepro-von Willebrand factor. J Biol Chem. 269. 2440-2446
  • Gum, JR Jr, Ho, JJ, Pratt, WS, Hicks, JW, Hill, AS, Vinall, LE. (1997). MUC3 human intestinal mucin: Analysis of gene structure, the carboxyl terminus and a novel upstream repetitive region. J Biol Chem. 272. 26678-26686
  • Moniaux, N, Nollet, S, Porchet, N, Degand, P, Laine, A, Aubert, JP. (1999). Complete sequence of the human mucin MUC4: a putative cell membrane-associated mucin. Biochem J. 338. 325-333
  • Mehrotra, R, Thornton, DJ, Sheehan, JK. (1998). Isolation and physical characterization of the MUC7 (MG2) mucin from saliva: evidence for self-association. Biochem J. 334. 415-422
  • Meezaman, D, Charles, P, Daskal, E, Polymeropoulos, MH, Martin, BM, Rose, MC. (1994). Cloning and analysis of cDNA encoding a major airway glycoprotein, human tracheobronchial mucin (MUC5). J Biol Chem. 269. 12932-12939
  • Lapensee, L, Paquette, Y, Bleau, G. (1997). Allelic polymorphism and chromosomal localization of the human oviductin gene (MUC9). Fertil Steril. 68. 702-708
  • Williams, SJ, McGuckin, MA, Gotley, DC, Eyre, HJ, Sutherland, GR, Antalis, TM. (1999). Two novel mucin genes down-regulated in colorectal cancer identified by differential display. Cancer Res. 59. 4083-4089
  • Williams, SJ, Wreschner, DH, Tran, M, Eyre, HJ, Sutherland, GR, McGuckin, MA. (2001). MUC13, a novel human cell surface mucin expressed by epithelial and hemopoietic cells. J Biol Chem. 276. 18327-18336
  • Pallesen, LT, Berglund, L, Rasmussen, LK, Petersen, TE, Rasmussen, JT. (2002). Isolation and characterization of MUC15, a novel cell membrane-associated mucin. Eur J Biochem. 269. 2755-2763
  • Yin, BW, Lloyd, KO. (2001). Molecular cloning of the CA125 ovarian cancer antigen: identification as a new mucin, MUC16. J Biol Chem. 276. 27371-27375
  • Gum, JR Jr, Crawley, SC, Hicks, JW, Szymkowski, DE, Kimp, YS. (2002). MUC17, a novel membrane-tethered mucin. Biochem Biophys Res Commun. 291. 466-475
  • Inatomi, T, Spurr-Michaud, S, Tisdale, AS, Gipson, IK. (1995). Human corneal and conjunctival epithelia express MUC1 mucin. Invest Ophthalmol Vis Sci. 36. 1818-1827
  • Inatomi, T, Spurr-Michaud, S, Tisdale, AS, Zhan, Q, Feldman, ST, Gipson, IK. (1996). Expression of secretory mucin genes by human conjunctival epithelia. Invest Ophthalmol Vis Sci. 37. 1684-1692
  • Berry, M, Ellingham, RB, Corfield, AP. (1996). Polydispersity of normal human conjunctival mucins. Invest Ophthalmol Vis Sci. 37. 2559-2571
  • Jumblatt, MM, McKenzie, RW, Jumblatt, JE. (1999). MUC5AC mucin is a component of the human precorneal tear film. Invest Ophthalmol Vis Sci. 40. 43-49
  • Pflugfelder, SC, Liu, Z, Monroy, D, Li, DQ, Carvajal, ME, Price-Schiavi, SA. (2000). Detection of sialomucin complex (MUC4) in human ocular surface epithelium and tear fluid. Invest Ophthalmol Vis Sci. 41. 1316-1326
  • McKenzie, RW, Jumblatt, JE, Jumblatt, MM. (2000). Quantification of MUC2 and MUC5AC transcripts in human conjunctiva. Invest Ophthalmol Vis Sci. 41. 703-708
  • Jumblatt, MM, McKenzie, RW, Steele, PS, Emberts, CG, Jumblatt, JE. (2003). MUC7 expression in the human lacrimal gland and conjunctiva. Cornea. 22. 41-45
  • Argueso, P, Balaram, M, Spurr-Michaud, S. (2002). Decreased levels of the goblet cell mucin MUC5AC in tears of patients with Sjogren syndrome. Invest Ophthalmol Vis Sci. 43. 1004-1011
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