Effect of mycoviruses on growth, spore germination and pathogenicity of the fungus Fusarium circinatumShort communication

  1. Flores-Pacheco, J. Asdrúbal
  2. Muñoz-Adalia, E. Jordán
  3. Martínez-Álvarez, Pablo
  4. Pando, Valentín
  5. Díez-Casero, Julio J.
  6. Martín-García, Jorge
Revue:
Forest systems

ISSN: 2171-5068

Année de publication: 2017

Volumen: 26

Número: 3

Type: Article

DOI: 10.5424/FS/2017263-11060 DIALNET GOOGLE SCHOLAR lock_openDialnet editor

D'autres publications dans: Forest systems

Objectifs de Développement Durable

Résumé

Aim of the study: To assess the impact on two mycoviruses recently described in F. circinatum mitovirus 1, 2-1 and 2-2 (FcMV1, FcMV2-1 and FcMV2-2) on i) mycelial growth, ii) spore germination and iii) relative necrosis.Material and methods: Fourteen monosporic strains of F. circinatum (one of each pair infected with mycoviruses and the other without them) of the pathogen with and without viruses were selected for the assay. The statistical analysis, were a linear mixed model of analysis of variance considering one between-subjects factor (isolate) and one within-subjects factor with four levels (1=without viruses, 2=only virus FcMV1, 3=only virus FcMV2-2 and 4=both viruses).Main results: Colony growth rates of F. circinatum isolates were significantly reduced in presence of mycoviruses (p=0.002). The spore germination was also reduced in the F. circinatum isolates containing mycovirus as compared to mycovirus-free isolates (p<0.001). No significant differences in lesion lengths caused by F. circinatum were found in relation to the presence/absence of mycovirus (p<0.61).Research highlights: Reduction of the percentage of spore germination in the isolates of F. circinatum with mycovirus infections, as compared to free isolates, provides indications of reduction of metabolic activity and plant physiology are discussed. The lack of significant differences found in the length of the lesions caused by F. circinatum with respect to the presence/absence of mycovirus, indicates that further studies with a larger number of variables are required.  

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Information sur le financement

The Ministry of Economy and Competitiveness of the Government of Spain (Projects AGL2012-39912 and AGL2015-69370-R); COST Action FP1406 PINESTRENGTH; Academic Mobility program for Inclusive Development in Latin America (AMIDILA)/Erasmus Mundus Action 2 in partnership with Bluefields Indian & Caribbean University (BICU) – Nicaragua (Scholarship to JAFP); Portuguese Foundation for Science and Technology (FCT) supported JMG (Post doc grant - SFRH/BPD/122928/2016).

Financeurs

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