Estandarización de Emergencia para el Diagnóstico del virus SARS-CoV-2 mediante la Reacción en Cadena de la Polimerasa de Transcripción Reversa en Tiempo Real (RT-PCR) en situación de pandemia de COVID-19

  1. Diego Fernández Lázaro
  2. Natalia Sanz Gómez
  3. Nerea Sánchez Serrano
  4. Assma Alaoui Sosse
  5. Carmen Aldea Mansilla
Journal:
Revista Madrileña de Salud Pública: REMASP

ISSN: 2659-9716

Year of publication: 2020

Volume: 4

Issue: 7

Pages: 1-11

Type: Article

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More publications in: Revista Madrileña de Salud Pública: REMASP

Abstract

In the face of the emerging coronavirus pandemic SARS-CoV-2 (Severe Acute Respiratory Sindrome Coronavirus 2 related) accurate standardization of hospital diagno-sis is necessary to reduce the response time for con-firmation of a suspected case. For this reason, labora-tory diagnosis is a priority for public health systems. In the area of Soria, with 100,000 inhabitants, a very old population, a mortality rate double that of Spain for COVID-19, the Hospital Santa Bárbara of the public network is the only diagnostic center for SARS-CoV-2. In the Microbiology Service, we have established a ro-bust diagnostic methodology for the detection of viral RNA present in samples from infected patients using the Real-Time Reverse Transcription Polymerase Chain Reaction (RT-PCR) technique, which allows a report to be issued in approximately 4 hours. This study descri-bes the process that could guide the Microbiology Ser-vices of other Hospitals

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