Phaseolus lunatus is nodulated by a phosphate solubilizing strain of Sinorhizobium meliloti in a Peruvian soil

  1. Ormeño, E. 1
  2. Torres, R. 1
  3. Mayo, J. 1
  4. Rivas, R. 3
  5. Peix, A. 2
  6. Velázquez, E. 3
  7. Zúñiga, D. 1
  1. 1 Universidad Nacional Agraria La Molina
    info

    Universidad Nacional Agraria La Molina

    Lima, Perú

    ROR https://ror.org/00vr49948

  2. 2 Instituto de Recursos Naturales y Agrobiología de Salamanca
    info

    Instituto de Recursos Naturales y Agrobiología de Salamanca

    Salamanca, España

    ROR https://ror.org/051p0fy59

  3. 3 Universidad de Salamanca
    info

    Universidad de Salamanca

    Salamanca, España

    ROR https://ror.org/02f40zc51

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Actas:
First International Meeting on Microbial Phosphate Solubilization
  1. E. Velázquez (coord.)
  2. C. Rodríguez-Barrueco (coord.)

Editorial: Springer Dordrecht

ISBN: 978-1-4020-4019-1,  978-90-481-7013-5,  978-1-4020-5765-6

Año de publicación: 2007

Páginas: 143-147

Congreso: First International Meeting on Microbial Phosphate Solubilization,Salamanca, Spain, July 16–19, 2002

Tipo: Aportación congreso

DOI: 10.1007/978-1-4020-5765-6_21 GOOGLE SCHOLAR

Objetivos de desarrollo sostenible

Resumen

The genus Phaseolus includes several species indigenous to American continent that belong to family Leguminosae. This genus includes several species, some of them only cultivated in American countries. This is the case of Phaseolus lunatus. This plant can be nodulated by fast and slow growing rhizobia. At the moment the fast growing species nodulating Phaseolus commonly belong to genus Rhizobium and more rarely to Sinorhizobium fredii. A strain, LMTR32, isolated from Phaseolus lunatus growing in Peru soils showed a high ability to solubilize bicalcium phosphate from YED-P plates. The 16S rRNA sequence of this strain showed a 100% similarity with the type strain of Sinorhizobium meliloti. The LMW RNA profile of this strain is identical to that of type strain of Sinorhizobium meliloti and confirms that the strain LMTR32 belongs to this species. More studies are necessary in order to establish the prevalence of this species in nodules of Phaseolus lunatus in Perú, and, in the future, it will be very interesting to perform wider taxonomic studies of rhizobia nodulating Phaseolus in different American countries.

Referencias bibliográficas

  • Amarger N, Macheret V and Laguerre G 1997 Rhizobium gallicum sp. nov. and Rhizobium giardinii sp. nov. from Phaseolus vulgaris nodules. Int. J. Syst. Bacteriol. 47, 996–1006.
  • Bergersen F J 1961 The growth of Rhizobium in synthetic media. Aust. J. Biol. Sci. 14, 349–360.
  • Beringer J E 1974 R factors transfer in Rhizobium leguminosarum. J. Gen. Microbiol. 84, 188–198.
  • Bidle K D and Fletcher M 1995 Comparison of free-living and particle-associated bacterial communities in the Chesapake Bay by stable low-molecular-weight RNA analysis. Appl. Environ. Microbiol. 61, 944–952.
  • Cruz-Sánchez J M, Velázquez E, Mateos P and Martínez-Molina E 1997 Enhancement of resolution of low molecular weight RNA profiles by staircase electrophoresis. Electrophoresis 18, 1909–1911.
  • de Freitas J R, Banerjee M R and Germida J J 1997 Phosphate-solubilizing rhizobacteria enhance the growth and yield but not phosphorous uptake in canola (Brassica napus L.). Biol. Fertil. Soils 24, 358–364.
  • Haas H, Budowle B and Weiler G 1994 Horizontal polyacrylamide gel electrophoresis for the separation of DNA fragments. Electrophoresis 15, 153–158.
  • Halder A K, Mishra A K and Chakrabartty P K 1990 Solubilization of phosphatic compounds by Rhizobium. Indian J. Microbiol. 30, 311–314.
  • Halder A K, Mishra A K, Bhattacharyya P and Chakrabartty P K 1993 Solubilization of rock phosphate by Rhizobium and Bradyrhizobium. J. Gen. Appl. Microbiol. 36, 81–92.
  • Herrera-Cervera J A, Caballero-Mellado J, Laguerre G, Tichy H V, Requena N, Amarger N, Martínez-Romero E, Olivares J and Sanjuán J 1999 At least five rhizobial species nodulate Phaseolus vulgaris in a Spanish soil. FEMS Microbiol. Ecol. 30, 87–97.
  • Höfle M G 1988 Identification of bacteria by low molecular weight RNA profiles: A new chemotaxonomic approach. J. Microbiol. Meth. 8, 235–248.
  • Jonhson F J 1990 Detection method of nitrogen (total) in fertilizers. In Methods of Analysis of the Association of Official Analytical Chemists. Ed. K Elrich. pp. 17–19. Association of Official Analytical Chemists, USA.
  • Jordan D C 1984 Family III, Rhizobiaceae Conn 1938 321AL. In Bergeýs Manual of Systematic Bacteriology Vol. 1. Eds. N R Krieg and J G Holt. pp. 234–244. Williams & Wilkins, Baltimore.
  • Martínez-Romero E, Segovia L, Mercante F M, Franco A A, Graham P and Pardo M A 1991 Rhizobium tropici, a novel species nodulating Phaseolus vulgaris L. beans and Leucaena sp. trees. Int. J. Syst. Bacteriol. 41, 417–426.
  • Laguerre G, Nour S M, Macheret V, Sanjuán J, Drouin P and Amarguer N 2001 Classification of rhizobia based on nodC and nifH gene analysis reveals a close phylogenetic relationship among Phaseolus vulgaris symbionts. Microbiology 147, 981–993.
  • Matos G and Zúñiga D 2002 Comportamiento de cepas nativas de rhizobios aisladas de la costa del Perúen dos cultivares de pallar (Phaseolus lunatus). Ecol. Appl. 1, 19–24.
  • Mhamdi R, Laguerre G, Elarbi-Aouani M, Mars M and Amarger N 2002 Different species and symbiotic genotypes of field rhizobia can nodulate Phaseolus vulgaris in Tunisian soils. FEMS Microbiol. Ecol. 41, 77–84.
  • Pearson W R and Lipman D J 1988 Improved tools for biological sequence comparison. Proc. Nat. Acad. Sci. USA 85, 2444–2448.
  • Peix A., Rivas-Boyero A.A., Mateos P.F., Rodríguez-Barrueco C., Martínez-Molina E. and Velázquez E 2001 Growth promotion of chickpea and barley by a phosphate solubilizing strain of Mesorhizobium mediterraneum under growth chamber conditions. Soil Biol. Biochem. 33, 103–110.
  • Rivas R, Velázquez E, Valverde A, Mateos P F and Martínez-Molina E 2001 A two primers random amplified polymorphic DNA procedure to obtain polymerase chain reaction fingerprints of bacterial species. Electrophoresis 22, 1086–1089.
  • Rivas R, Velázquez E, Willems A, Vizcaíno N, Subba-Rao N S, Mateos P F, Gillis M, Dazzo F B and Martínez-Molina E 2002 A new species of Devosia that forms a nitrogen-fixing root-nodule symbiosis with the aquatic legume Neptunia natans (L. f.) Druce. Appl. Environ. Microbiol. 68, 5217–5222.
  • Rodríguez-Navarro D N, Buendía A M, Camacho M, Lucas M and Santamaría C 2000 Characterization of Rhizobium spp. bean isolates from southwest of Spain. Soil. Biol. Biochem. 32, 1601–1613.
  • Sadowsky M J, Cregan P and Keyser H H 1988 Nodulation and nitrogen fixation efficacy of Rhizobium fredii with Phaseolus vulgaris genotypes. Appl. Environ. Microbiol. 54, 1907–1910.
  • Segovia L, Young J P W and Martínez-Romero E 1993 Reclassification of American Rhizobium leguminosarum biovar phaseoli type I strains as Rhizobium etli sp. nov. Int. J. Syst. Bacteriol. 43, 374–377.
  • Sprinzl M, Moll J, Meissner F and Hatmann T 1985 Compilation of tRNA sequences. Nucleic Acid Res. 13, 1–49.
  • van Berkum P, Beyene D, Bao G, Campbell T A and Eardly B D 1998 Rhizobium mongolense sp. nov. is one of three rhizobial genotypes identified which nodulate and form nitrogen-fixing symbioses with Medicago ruthenica [(L.) Ledebour]. Int. J. Syst. Bacteriol. 48, 13–22.
  • Velázquez E, Martínez-Romero E, Rodríguez-Navarro D N, Trujillo M E, Daza A, Mateos P F, Martinez-Molina E and Van Berkum P 2001a Characterization of rhizobial isolates of Phaseolus vulgaris by staircase electrophoresis of low molecular weight RNA. Appl. Environ. Microbiol. 67, 1008–1010.
  • Velázquez E, Trujillo M E, Peix A, Palomo J L, García-Benavides P, Mateos P, Ventosa A and Martínez-Molina E 2001b Stable low molecular weight RNA analyzed by staircase electrophoresis, a molecular signature for both prokaryotic and eukaryotic microorganisms. Syst. Appl. Microbiol. 24, 490–499.
  • Vincent J M 1970 The cultivation, isolation and maintenance of rhizobia. In A Manual for the Practical Study of Root-Nodule. Ed. J M Vincent. pp. 1–13. Blackwell Scientific Publications, Oxford.