Localización de los mRNA de los dos tipos de somatolactina en hipófisis de dorada
- M.J. Herrero-Turrión 1
- A. Velasco 1
- R. Rodríguez 1
- J. Aijón 1
- j. Lara 1
- 1 Instituto de Neurociencias de Castilla y León (INCyL), Univ. de Salamanca
ISSN: 1578-4541
Ano de publicación: 2001
Número: 13
Tipo: Artigo
Outras publicacións en: AquaTIC: revista electrónica de acuicultura
Resumo
Localization of mRNA of the two types of somatolactin in the pituitary of gilthead sea bream The expression of mRNA of the two types of somatolactin (SL1 and SL2) which have been found, up to the present, in the pituitary of gilthead sea bream (Sparus aurata) has been analysed by in situ hybridization (ISH). The method of non-radioactive ISH which we optimise in this study uses oligonucleotides labelled in extreme 5’ with biotin or dioxygenin as probes, which allows simple and double ISH of high specificity and sensitivity to be performed. The distinct probes of SL’s used present a strong signal fundamentally in the pars intermedia (PI) of the pituitary gland. This study detects cells which co-express both forms for the first time. Moreover, in the study of three groups of different sizes, produced by asynchronic growth of the bream in industrial cultivation, we did not find qualitative differences in the levels of expression of the GH/PRL gene family.
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