Molecular Responses to Solvent Stress: Strategies for Living in Unpalatable Substrates

  1. Ramos, Juan Luis
  2. Daniels, Craig
  3. Krell, Tino
  4. Duque, Estrella
  5. Godoy, Patricia
  6. de la Torre, Jesús
  7. Fernández-Escamilla, Ana M.
  8. Daddaoua, Abdelali
  9. Navarro-Avilés, Gloria
  10. Fillet, Sandy
  11. Pini, Cecilia
  12. Molina-Henares, M. Antonia
  13. Lacal, Jesús
  14. Busch, Andreas
  15. Silva-Jiménez, Hortencia
  16. Rodríguez, Sara
  17. Molina, Lázaro
  18. Bursakov, Sergey A.
  19. Roca, Amalia
  20. Segura, Ana
Liburua:
Extremophiles Handbook

Argitaletxea: Springer, Tokyo

ISBN: 978-4-431-53897-4 978-4-431-53898-1

Argitalpen urtea: 2011

Orrialdeak: 971-990

Mota: Liburuko kapitulua

DOI: 10.1007/978-4-431-53898-1_47 GOOGLE SCHOLAR lock_openSarbide irekia editor

Garapen Iraunkorreko Helburuak

Laburpena

The Extremophiles Handbook brings together the rapidly growing and often scattered information on microbial life in the whole range of extreme environments. This book will be a useful reference for finding clues to the origin of life and for exploring the biotechnology potential of these fascinating organisms.

Erreferentzia bibliografikoak

  • Akama H, Matsuura T, Kashiwagi S, Yoneyama H, Narita S, Tsukihara T, Nakagawa A, Nakae T (2004) Crystal structure of the membrane fusion protein, MexA, of the multidrug transporter in Pseudomonas aeruginosa. J Biol Chem 279:25939–25942
  • Alguel Y, Meng C, Terán W, Krell T, Ramos JL, Gallegos MT, Zhang X (2007) Crystal structure of multidrug binding protein TtgR in complex with antibiotics and plant antimicrobials. J Mol Biol 369:829–840
  • Aono R, Ito M, Inoue A, Horikoshi H (1992) Isolation of a novel toluene-tolerant strain of Pseudomonas aeruginosa. Biosci Biotechnol Biochem 1:145–146
  • Aono R, Tsukagoshi N, Yamamoto M (1998) Involvement of outer membrane protein TolC, a possible member of the mar-sox regulon, in maintenance and improvement of organic solvent tolerance of Escherichia coli K-12. J Bacteriol 180:938–944
  • Bernal P, Muñoz-Rojas J, Hurtado A, Ramos JL, Segura A (2007a) A Pseudomonas putida cardiolipin synthesis mutant exhibits increased sensitivity to drugs related to transport functionality. Environ Microbiol 9:1135–1145
  • Bernal P, Segura A, Ramos JL (2007b) Compensatory role of the cis-trans isomerase and cardiolipin synthase in the membrane fluidity of Pseudomonas putida DOT-T1E. Environ Microbiol 9:1658–1664
  • Bugg T, Foght JM, Pickard MA, Gray MR (2000) Uptake and active efflux of polycyclic aromatic hydrocarbons by Pseudomonas fluorescens LP6a. Appl Environ Microbiol 66:5387–5392
  • Cruden DL, Wolfram JH, Rogers RD, Gibson DT (1992) Physiological properties of a Pseudomonas strain which grows with p-xylene in a two-phase (organic-aqueous) medium. Appl Environ Microbiol 58:2723–2729
  • Daniels C, Daddaoua A, Lu D, Zhang X, Ramos JL (2010) Domain cross-talk during effector binding to the multidrug binding TtgR regulator. J Biol Chem 285:21372–21381
  • Dawson RJP, Locher KP (2006) Structure of a bacterial multidrug ABC transporter. Nature 443:180–185
  • Diefenbach R, Keweloh H (1994) Synthesis of trans unsaturated fatty acids in Pseudomonas putida P8 by direct isomerisation of the double bond of lipids. Arch Microbiol 162:120–125
  • Diefenbach R, Heipieper HJ, Keweloh H (1992) The conversion of cis- into trans-unsaturated fatty acids in Pseudomonas putida P8: evidence for a role in the regulation of membrane fluidity. Appl Microbiol Biotechnol 38:382–387
  • Domínguez-Cuevas P, González-Pastor JE, Marqués S, Ramos JL, de Lorenzo V (2006) Transcriptional trade-off between metabolic and stress-response programs in Pseudomonas putida KT2440 cells exposed to toluene. J Biol Chem 281:11981–11991
  • Dong Y-H, Wang L-H, Zhang L-H (2007) Quorum-quenching microbial infections: mechanisms and implications. Philos Trans R Soc Lond B 362:1201–1211
  • Duque E, Segura A, Mosqueda G, Ramos JL (2001) Global and cognate regulators control the expression of the organic solvent efflux pumps TtgABC and TtgDEF of Pseudomonas putida. Mol Microbiol 39:1100–1106
  • Duque E, Rodríguez-Herva J-J, de la Torre J, Domínguez-Cuevas P, Múñoz-Rojas J, Ramos JL (2007) The RpoT regulon of Pseudomonas putida DOT-T1E and its role in stress endurance against solvents. J Bacteriol 189:207–219
  • Eda S, Maseda H, Nakae T (2003) An elegant means of self-protection in gram-negative bacteria by recognizing and extruding xenobiotics from the periplasmic space. J Biol Chem 278:2085–2088
  • Eswaran J, Koronakis E, Higgins MK, Hughes C, Koronakis V (2004) Three’s company: component structures bring a closer view of tripartite drug efflux pumps. Curr Opin Struct Biol 14:741–747
  • Evans K, Adewage L, Poole K (2001) MexR repressor of the mexAB-oprM multidrug efflux operon of Pseudomonas aeruginosa: identification of MexR binding sigres in the mexA-mexR intergenic region. J Bacteriol 183:807–812
  • Fernandez-Recio J, Walas F, Federici L, Venkatesh Pratap J, Bavro VN, Miguel RN, Mizuguchi K, Luisi B (2004) A model of a transmembrane drug-efflux pump from Gram-negative bacteria. FEBS Lett 578:5–9
  • Fillet S, Vélez M, Lu D, Zhang X, Gallegos MT, Ramos JL (2009) TtgV Represses two different promoters by recognizing different sequences. J Bacteriol 191:1901–1909
  • Fralick JA (1996) Evidence that TolC is required for functioning of the Mar/AcrAB efflux pump of Escherichia coli. J Bacteriol 178:8503–8505
  • Fukumori F, Hirayama H, Takami H, Inoue A, Horikoshi K (1998) Isolation and transposon mutagenesis of a Pseudomonas putida KT2442 toluene-resistant variant: involvement of an efflux system in solvent resistance. Extremophiles 2:395–400
  • García V, Godoy P, Daniels C, Hurtado A, Ramos JL, Segura A (2009) New transporters involved in stress tolerance in Pseudomonas putida DOT-T1E: from proteomic and transcriptomic data to functional analysis. Environ Microbiol. Reports 2:389–395
  • Ge Q, Yamada Y, Zgurskaya H (2009) The C-terminal domain of AcrA is essential for the assembly and function of the multidrug efflux pump AcrAB-TolC. J Bacteriol 191:4365–4371
  • Godoy P, Molina-Henares AJ, de la Torre J, Duque E, Ramos JL (2010) Characterization of RND efflux pumps: in silico to in vivo confirmation of four functionally distinct groups. Microb Biotechnol Doi:10.1111/j1751-7915.2010.00189x
  • Grogan DW, Cronan JE Jr (1997) Cyclopropane ring formation in membrane lipids of bacteria. Microbiol Mol Biol Rev 61:429–441
  • Guazzaroni ME, Terán W, Zhang X, Gallegos MT, Ramos JL (2004) TtgV bound to a complex operator site represses transcription of the single promoter for the multidrug and solvent extrusion TtgGHI pump. J Bacteriol 186:2921–2927
  • Guazzaroni ME, Krell T, Felipe A, Ruíz R, Meng C, Zhang X, Gallegos MT, Ramos JL (2005) The multidrug efflux regulator TtgV recognizes a wide range of structurally different effectors in solution and complexed with target DNA. Evidence from isothermal titration calorimetry. J Biol Chem 280:20887–20893
  • Guazzaroni ME, Krell T, Gutierrez del Arroyo P, Vélez M, Jiménez M, Rivas G, Ramos JL (2007) The transcriptional repressor TtgV recognizes a complex operator as a tetramer and induces convex DNA bending. J Mol Biol 369:927–939
  • Hamamoto T, Takata N, Kudo T, Horikoshi K (1994) Effect of temperature and growth phase on fatty acid composition of the psychrophilic Vibrio sp. Strain no.5710. FEMS Microbiol Lett 119:77–81
  • Härtig C, Loffhagen N, Harms H (2005) Formation of trans fatty acids is not involved in growth-linked membrane adaptation of Pseudomonas putida. Appl Environ Microbiol 71:1915–1922
  • Hearn EM, Dennis JJ, Gray MR, Foght JM (2003) Identification and characterization of the emhABC efflux system for polycyclic aromatic hydrocarbons in Pseudomonas fluorescens cLP6a. J Bacteriol 185:6233–6240
  • Hearn EM, Gray MR, Foght JJ (2006) Mutations in the central cavity and periplasmi domain affect efflux activity of the Resitance-Nodulation-Division pump EmhB from Pseudomonas fluorescens Lp6a. J Bacteriol 188:115–123
  • Heipieper HJ, Diefenbach R, Keweloh H (1992) Conversion of cis unsaturated fatty acids to trans, a possible mechanism for the protection of phenol-degrading Pseudomonas putida P8 from substrate toxicity. Appl Environ Microbiol 58:1847–1852
  • Heipieper HJ, Meulenbeld G, VanOirschot Q, de Bont JAM (1996) Effect of the environmental factors on the trans/cis ratio of unsaturated fatty acids in Pseudomonas putida S12. Appl Environ Microbiol 62:2773–2777
  • Heipieper HJ, de Waard P, van der Meer P, Killian JA, Isken S, de Bont JAM, Eggink GA, de Wolf FA (2001) Regiospecific effect of 1-octanol on cis-trans isomerisation of unsaturated fatty acids in the solvent-tolerant strain Pseudomonas putida S12. Appl Microbiol Biotechnol 57:541–547
  • Heipieper HJ, Meinhardt F, Segura A (2003) The cis-trans isomerise of unsaturated fatty acids in Pseudomonas and Vibrio: biochemistry, molecular biology and physiological function of a unique stress adaptive mechanism. FEMS Microbiol Lett 229:1–7
  • Hernández-Mendoza A, Quinto C, Segovia L, Pérez-Rueda E (2007) Ligand-binding prediction in the resistance-nodulation-cell division (RND) proteins. Comput Biol Chem 31:115–123
  • Hinrichs W, Kisker C, Duvel M, Muller A, Tovar K, Hillen W, Saenger W (1994) Structure of the Tet repressor-tetracycline complex and regulation of antibiotic resistance. Science 264:418–420
  • Holtwick R, Keweloh H, Meinhardt F (1999) Cis/trans isomerase of unsaturated fatty acids of Pseudomonas putida P8: evidence for a heme protein of the cytochromo c type. Appl Environ Microbiol 65:2644–2649
  • Huertas MJ, Duque E, Molina L, Roselló-Mora R, Mosqueda G et al (2000) Tolerance to sudden organic solvent shocks by soil bacteria and characterization of Pseudomonas putida strains isolated from toluene polluted sites. Environ Sci Technol 34:3395–3400
  • Ingram LO (1986) Microbial tolerance to alcohols: role of the cell membrane. Trends Biotechnol 4:40–44
  • Inoue A, Korikoshi K (1989) A Pseudomonas thrives in high concentrations of toluene. Nature 338:264–266
  • Isken S, de Bont JAM (1996) Active efflux of toluene in a solvent-resistant bacterium. J Bacteriol 178:6056–6058
  • Ju K-S, Parales R (2006) Control of substrate specificity by active site residues in nitrobenzene dioxygenase. Appl Environ Microbiol 72:1817–1824
  • Junker F, Ramos JL (1999) Involvemente of the cis/trans isomerase Cti in solvent resistance of Pseudomonas putida DOT-T1E. J Bacteriol 181:5693–5700
  • Kallimanis AS, Frillingos KD, Koukkou AI (2007) Taxonomic identification, phenanthreen uptake activity, and membrane lipid alterations of the PAH degrading Arthrobacter sp. strain Sphe3. Appl Microbiol Biotechnol 76:709–717
  • Kieboom J, Dennis JJ, Zylstra GJ, de Bont JAM (1998a) Active efflux of organic solvents by Pseudomonas putida S12 is induced by solvents. J Bacteriol 180:6769–6772
  • Kieboom J, Dennis JJ, de Bont JA, Zylstra GJ (1998b) Identification and molecular characterization of an efflux pump involved in Pseudomonas putida S12 solvent tolerance. J Biol Chem 273:85–91
  • Kieboom J, Bruinenberg R, Keizer-Gunnink I, de Bont JAM (2001) Transposon mutations in the flagella biosynthetic pathway of the solvent-tolerant Pseudomonas putida S12 result in a decreased expression of solvent efflux genes. FEMS Microbiol Lett 198:117–122
  • Kim K, Lee S, Lee K, Lim D (1998) Isolation and characterization of toluene-sensitive mutants from the toluene-resistant bacterium Pseudomonas putida GM73. J Bacteriol 180:3692–3696
  • Kobayashi K, Tsukagoshi N, Aono R (2001) Suppression of hypersensitivity of Escherichia coli acrB mutant to organic solvents by integrational activation of the acrEF operon with the IS1 or IS2 element. J Bacteriol 183:2646–2653
  • Köhler T, Epp SF, Kocjancic Curty L, Pechère J-C (1999) Characterization of MexT, the regulator of the MexE-MexF-OprN multidrug efflux system of Pseudomonas aeruginosa. J Bacteriol 181:6300–6305
  • Koronakis V, Sharff A, Koronakis E, Luisi B, Hughes C (2000) Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export. Nature 405:914–919
  • Krell T, Molina-Henares AJ, Ramos JL (2006) The IclR family of transcriptional activators and repressors can be defined by a single profile. Protein Sci 15:1207–1213
  • Lee A, Mao W, Warren MS, Mistry A, Hoshino K et al (2000) Interplay between efflux pumps may provide either additive of multiplicative effects on drug resistance. J Bacteriol 182:3142–3150
  • Li X, Zhang L, Poole K (1998) Role of the multidrug efflux systems of Pseudomonas earuginosa in organic solvent tolerance. J Bacteriol 180:2987–2991
  • Ma D, Cook DN, Alberti M, Pon NG, Nikaido H, Hearst JE (1993) Molecular cloning and characterization of acrA and acrE genes of Escherichia coli. J Bacteriol 175:6299–6313
  • Mashburn-Warren L, Howe J, Brandenburg K, Whiteley M (2009) Structural requirements of the Pseudomonas quinolone signal for membrane vesicle stimulation. J Bacteriol 191:3411–3414
  • Masuda N, Sakagawa E, Ohya S, Gotoh N, Tsujimoto H, Nishino T (2000) Substrate specificities of MexAB-OprM, MexCD-OprJ, and MexXY-oprM efflux pumps in Pseudomonas aeruginosa. Antimicrob Agents Chemother 44:3322–3327
  • Molina-Henares AJ, Krell T, Guazzaroni ME, Segura A, Ramos JL (2006) Members of the IclR family of bacterial transcriptional regulators function as activators and/or repressors. FEMS Microbiol Rev 30:157–186
  • Mosqueda G, Ramos JL (2000) A set of genes encoding a second toluene efflux system in Pseudomonas putida DOT-T1E is linked to the tod genes for toluene metabolism. J Bacteriol 182:937–943
  • Mosqueda G, Ramos-González MI, Ramos JL (1999) Toluene metabolism by the solvent-tolerant Pseudomonas putida DOT-T1 strain, and its role in solvent impermeabilization. Gene 232:69–76
  • Muñoz-Rojas J, Bernal P, Duque E, Godoy P, Segura A, Ramos JL (2006) Involvement of cyclopropane fatty acids in the response of Pseudomonas putida KT2440 to freeze-drying. Appl Environ Microbiol 72:472–477
  • Murakami S, Nakashima R, Yamashita E, Matsumoto T, Yamaguchi A (2006) Crystal structures of a multidrug transporter reveal a functionally rotating mechanism. Nature 443:173–179
  • Neumann G, Kabelitz N, Zehnsdorf A, Miltner A, Lippold H, Meyer D, Schmid A, Heipieper HJ (2005) Prediction of the adaptability of Pseudomonas putida DOT-T1E to a second phase of a solvent for economically sound two-phase biotransformations. Appl Environ Microbiol 71:6606–6612
  • Neumann G, Cornelissen S, Van Breukelen F, Hunger S, Lippold H, Lofhagen N, Wick LY, Heipieper HJ (2006) Energetics and surface properties of Pseudomonas putida DOT-T1E in a two-phase fermentation system with 1-decanol as second phase. Appl Environ Microbiol 72:4232–4238
  • Nikaido H (1996) Multidrug efflux pumps of gram-negative bacteria. J Bacteriol 178:5853–5859
  • Okuyama H, Okajima N, Sasaki S, Higashi S, Murata N (1991) The cis/trans isomerisation of the double bond of a fatty acid as a strategy for adaptation to hcanges in ambient temperature in the psychrophilic bacterium, Vibrio sp. strain ABE-1. Biochim Biophys Acta 1084:13–20
  • Okuyama H, Ueno A, Enari D, Morita N, Kusano T (1998) Purification and characterization of 9-hexadecenoic acid cis-trans isomerase from Pseudomonas sp. strain E-3. Arch Microbiol 169:29–35
  • Pearson JP, van Delden C, Iglewski BH (1999) Active efflux and diffusion are involved in transport of Pseudomonas aeruginosa cell-to-cell signals. J Bacteriol 181:1203–1210
  • Pedrotta V, Witholt B (1999) Isolation and characterization of the cis-trans unsaturated fatty acid isomerase of Pseudomonas oleovorans GPo12. J Bacteriol 181:3256–3261
  • Phoenix P, Keane A, Patel A, Bergeron H, Ghoshal S, Lau PC (2003) Characterization of a new solvent-responsive gene locus in Pseudomonas putida F1 and its functionalization as a versatile biosensor. Environ Microbiol 5:1309–1327
  • Pini CV, Bernal P, Godoy P, Ramos JL, Segura A (2009) Cyclopropane fatty acids are involved in organic solvent tolerance but not in acid stress resistance in Pseudomonas putida DOT-T1E. Microb Biotechnol 2:253–261
  • Pinkart HC, Wolfram JW, Rogers R, White DC (1996) Cell envelope changes in solvent-tolerant and solvent-sensitive Pseudomonas putida strains following exposure to o-xylene. Appl Environ Microbiol 62:1129–1132
  • Poole K (2004) Efflux pumps. In: Ramos JL (ed) Pseudomonas: genomics, life style and molecular architecture, chapter 21, vol 1. Kluwer, New York, pp 635–674
  • Ramos JL, Duque E, Huertas MJ, Haïdour A (1995) Isolation and expansion of the catabolic potential of a Pseudomonas putida strain able to grow in the presence of high concentrations of aromatic hydrocarbons. J Bacteriol 177:3911–3916
  • Ramos JL, Duque E, Rodriguez-Hervá JJ, Godoy P, Haidour A, Reyes F, Fernandez-Barrero A (1997) Mechanisms for solvent tolerance in bacteria. J Biol Chem 272:3887–3890
  • Ramos JL, Duque E, Godoy P, Segura A (1998) Efflux pumps involved in toluene tolerance in Pseudomonas putida DOT-T1E. J Bacteriol 180:3323–3329
  • Ramos JL, Duque E, Gallegos M-T, Godoy P, Ramos-González MI, Rojas A, Terán W, Segura A (2002) Mechanisms of solvent tolerance in Gram-negative bacteria. Annu Rev Microbiol 56:743–768
  • Ramos JL, Martinez-Bueno M, Molina-Henares AJ, Teran W, Watanabe K, Zhang XD et al (2005) The TetR family of transcriptional repressors. Microbiol Mol Biol Rev 69:326–356
  • Ramos-González MI, Ben-Bassat A, Campos MJ, Ramos JL (2003) Genetic engineering of a highly solvent-tolerant Pseudomonas putida strain for biotransformation of toluene to p-hydroxybenzoate. Appl Environ Microbiol 69:5120–5127
  • Rodríguez-Herva JJ, García V, Hurtado A, Segura A, Ramos JL (2007) The ttgGHI solvent efflux pump operon of Pseudomonas putida DOT-T1E is located on a large self-transmissible plasmid. Environ Microbiol 9:1550–1561
  • Rojas A, Duque E, Mosqueda G, Golden G, Hurtado A, Ramos JL, Segura A (2001) Three efflux pumps are required to provide efficient tolerance to toluene in Pseudomonas putida DOT-T1E. J Bacteriol 183:3967–3973
  • Rojas A, Segura A, Guazzaroni ME, Teran W, Hurtado A, Gallegos MT, Ramos JL (2003) In vivo and in vitro evidence that TtgV is the specific regulator of the TtgGHI multidrug and solvent efflux pump of Pseudomonas putida. J Bacteriol 185:4755–4763
  • Rojas A, Duque E, Schmid A, Hurtado A, Ramos JL, Segura A (2004) Biotransformation in double-phase systems: physiological responses of Pseudomonas putida DOT-T1E to a double phase made of aliphatic alcohols and biosynthesis of substituted catechols. Appl Environ Microbiol 70:3637–3643
  • Ruhl J, Schmid A, Blank LM (2009) Selected Pseudomonas putida strains able to grow in the presence of high butanol concentrations. Appl Environ Microbiol 75:4653–4656
  • Schumacher MA, Miller MC, Grkovic S, Brown MH, Skurray RA, Brennan RG (2002) Structural basis for cooperative DNA binding by two dimers of the multidrug-binding protein QacR. EMBO J 21:1210–1218
  • Segura A, Duque E, Hurtado A, Ramos JL (2001) Mutations in genes involved in the flagellar export apparatus of the solvent-tolerant Pseudomonas putida DOT-T1E strain impair motility and lead to hypersensibility to toluene shocks. J Bacteriol 183:4127–4133
  • Segura A, Duque E, Rojas A, Godoy P, Delgado A, Hurtado A, Cronan JE Jr, Ramos JL (2004a) Fatty acid biosynthesis is involved in solvent tolerance in Pseudomonas putida DOT-T1E. Environ Microbiol 6:416–423
  • Segura A, Hurtado A, Duque E, Ramos JL (2004b) Transcriptional phase variation at the flhB gene of Pseudomonas putida DOT-T1E is involved in response to environmental changes and suggests the participation of the flagellar export system in solvent tolerance. J Bacteriol 186:1905–1909
  • Segura A, Godoy P, van Dillewijn P, Hurtado A, Arroyo N, Santacruz S, Ramos JL (2005) Proteomic analysis reveals the participation of energy-and stress-related proteins in the response of Pseudomonas putida DOT-T1E to toluene. J Bacteriol 187:5937–5945
  • Sikkema J, de Bont JA, Poolman B (1995) Mechanisms of membrane toxicity of hydrocarbons. Microbiol Rev 59:201–222
  • Sinensky M (1974) Homeoviscous adaptation – a homeostatic process that regulates the viscosity of membrane lipids in Escherichia coli. Proc Natl Acad Sci USA 71:522–525
  • Skindersoe ME, Alhede M, Phipps R, Yang L, Jensen PO, Rasmussen TB, Bjarnsholt T, Tolker-Nielsen T, Hoiby N, Givskov M (2008) Effects of antibiotics on quorum sensing in Pseudomonas aeruginosa. Antimicrob Agents Chemother 52:3648–3663
  • Srikumar R, Paul CJ, Poole K (2000) Influence of mutations in the mexR repressor gene on expression of the MexA-MexB-OprM multidrug efflux system of Pseudomonas aeruginosa. J Bacteriol 182:1410–1414
  • Su C-C, Rutherford DJ, Yu EW (2007) Characterization of the multidrug efflux regulator AcrR from Escherichia coli. Biochem Biophys Res Commun 361:85–90
  • Suutari T, Laakso S (1994) Microbial fatty-acids and thermal adaptation. Crit Rev Microbiol 20:129–137
  • Symmons MF, Bokma E, Koronakis E, Hughes C, Koronakis V (2009) The assembled structure of a complet tripartite bacterial multidrug efflux pump. Proc Natl Acad Sci USA 106:7173–7178
  • Terán W, Felipe A, Segura A, Rojas A, Ramos JL, Gallegos MT (2003) Antibiotic-dependent induction of Pseudomonas putida DOT-T1E TtgABC efflux pump is mediated by the drug binding repressor TtgR. Antimicrob Agents Chemother 47:3067–3072
  • Terán W, Krell T, Ramos JL, Gallegos MT (2006) Effector-repressor interactions, binding of a single effector molecule to the operator-bound TtgR homodimer mediates derepression. J Biol Chem 281:7102–7109
  • Terán W, Felipe A, Fillet S, Guazzaroni ME, Krell T, Ruiz R, Ramos JL, Gallegos MT (2007) Complexity in efflux pump control: cross-regulation by the paralogues TtgV and TtgT. Mol Microbiol 66:1416–1428
  • Tian T, Xu X-G, Duan H-M, Zhang L-Q (2010) The resistance nodualtion division efflux pump EmhABC influences the production of 2, 4-diacetylphloroglucinol in Pseudomonas fluorescens 2P24. Microbiology 156:39–48
  • Törnroth-Horsefield S, Gourdon P, Horsefield R, Brive L, Yamamoto N, Mori H, Snijder A, Neutze R (2007) Crystal structure of AcrB in complex with a single transmembrane subunit reveals another twist. Structure 15:1663–1673
  • Touzé T, Eswaran J, Bokma E, Koronakis E, Hughes C, Koronakis V (2004) Interactions underlying assembly of the Escherichia coli AcrAB-TolC multidrug efflux system. Mol Microbiol 53:697–706
  • Verhoef S, Wierckx N, Westerhof RGM, de Winde JH, Ruijssenaars HJ (2009) Bioproduction of p-hydroxystyrene from glucose by the solvent-tolerant bacterium Pseudomonas putida S12 in a two-phase water-decanol fermentation. Appl Environ Microbiol 75:931–936
  • Volkers RJM, de Long AdL, Huist AG, van Baar BLM, de Bont JAM, Wery J (2006) Chemostat-based proteomic analysis of toluene-affected Pseudomonas putida S12. Environ Microbiol 8:1674–1679
  • Von Wallbrunn A, Heipieper HJ, Meinhardt F (2002) Cis/trans isomerisation of unsaturated fatty acids in a cardiolipin synthase knock-out mutant of Pseudomonas putida P8. Appl Microbiol Biotechnol 60:179–185
  • Von Wallbrunn A, Richnow HH, Neumann G, Meinhardt FA, Heipieper HJ (2003) Mechanism of cis-trans isomerization of unsaturated fatty acids in Pseudomonas putida. J Bacteriol 185:1730–1733
  • Weber FJ, Ooijkaas LP, Schemen RM, Hartmans S, de Bont JA (1993) Adaptation of Pseudômonas putida S12 to high concentrations of styrene and other organic solvents. Appl Environ Microbiol 59:3502–3504
  • Weber FJ, Isken S, de Bont JAM (1994) Cis/trans isomerization of fatty acids as a defense mechanism of Pseudomonas putida strains to toxic concentrations of toluene. Microbiology 140:2013–2017
  • Wierckx NJP, Ballerstedt H, de Bont JAM, Wery J (2005) Engineering of solvent-tolerant Pseudomonas putida S12 for bioproduction of phenol from glucose. Appl Environ Microbiol 71:8221–8227
  • Wierckx NJP, Ballerstedt H, de Bont JAM, de Winde JH, Ruijssenaars HJ, Wery J (2008) Transcriptome analysis of a phenol-producing Pseudomonas putida S12 construct: genetic and physiological basis for improved production. J Bacteriol 190:2822–2830
  • Yu EW, McDermott G, Zgurskaya HI, Nikaido H, Koshland DE Jr (2003) Structural basis of multiple drug-binding capacity of the AcrB multidrug efflux pump. Science 300:976–980
  • Yu EW, Aires JR, McDermott G, Nikaido H (2005) A periplasmic drug-binding site of the AcrB multidrug efflux pump: a crystallographic and site-directed mutagenesis study. J Bacteriol 187:6804–6815
  • Zgurskaya HI, Nikaido H (1999) Bypassing the periplasm: reconstruction of the AcrAB multidrug efflux pump of Escherichia coli. Proc Natl Acad Sci USA 96:7190–7195
  • Zhu X, Long F, Chen Y, Knochel S, She Q, Shi X (2008) A putative ABC transporter is involved in negative regulation of biofilm formation by listeria monocytogenes. Appl Environ Microbiol 74:7675–7683
  • Ziha-Zarifi I, Llanes C, Köhler T, Pechère J-C, Plesiat P (1999) In vivo emergence of multidrug-resistant mutants of Pseudomonas aeruginosa overexpressing the active efflux system MexA-MexB-OprM. Antimicrob Agents Chemother 43:287–291