Preliminary study of ivermectin residues in bovine livers in the Bogota Savanna

  1. Carmen Teresa Celis-Giraldo 1
  2. Diego Ordóñez 1
  3. Leonardo Roa 1
  4. Sergio Andrei Cuervo-Escobar 1
  5. Dajane Garzón-Rodríguez 1
  6. Milena Alarcón-Caballero 1
  7. Luisa Fernanda Merchán 1
  1. 1 Universidad de Ciencias Aplicadas y Ambientales
    info

    Universidad de Ciencias Aplicadas y Ambientales

    Bogotá, Colombia

    ROR https://ror.org/01h2taq97

Revista:
Revista Mexicana de Ciencias Pecuarias

ISSN: 2448-6698,  2007-1124

Año de publicación: 2020

Volumen: 11

Número: 2

Páginas: 311-325

Tipo: Artículo

DOI: 10.22319/RMCP.V11I2.4992 DIALNET GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Revista Mexicana de Ciencias Pecuarias

Resumen

The present study tended to determine the ivermectin residues in cattle livers using the competitive ELISA technique and correlate gender and age variables with residual presence. Also, it was described the histopathological findings in analyzed samples. A total of 90 livers of randomly selected cattle were sampled in a type II slaughterhouse located in the Bogota Savanna. The samples were analyzed for ivermectin residues using the competitive ELISA technique and a histopathological evaluation was performed using the H&E technique. Only the 22 % (20/90) of the analyzed samples presented ivermectin residues. Of the positive individuals, the majority came from Cogua 35 % (7/20), Zipaquira 30 % (6/20) and Sopo 20 % (4/20). The breeds with residues presence corresponded to Half-Blood 35 % (7/20), Zebu 25 % (5/20), Normande 20 % (4/20) and Jersey x Holstein 15 % (3/20). Eighty five, 85 % (17/20) of the individuals were older than 1.5 yr. In regard to the gender variable, the majority of animals were males 65 % (13/20). Of the evaluated animals 3 % (3/90) exceeded the maximum residue limit (> 100 ppb). No association was found between the presence of residues and the gender and age variables (P>0.05). The majority of histopathological changes were mild or moderate, with alterations in architecture and inflammatory changes standing out. It was found association between the presence of residue and variables microcirculatory alteration, inflammatory alteration and changes similar to the cell death (P<0.05). As a conclusion, the competitive ELISA test used in this study served as a screening method for the detection ivermectin residues in the analyzed samples.

Referencias bibliográficas

  • Singh NK, Singh H, Jyoti, Haque M, Rath SS. Prevalence of parasitic infections in cattle of Ludhiana distric, Punjab. J Parasit Dis 2012;36(2):256-259.
  • Danaher M, Radeck W, Kolar L, Keegan J, Cerkvenik-Flajs V. Recent developments in the analysis of avermectin and milbemycin residues in food safety and the environment. Curr Pharm Biotechnol 2012;13:936-951.
  • Ộmura S. Ivermectin:25 years and still going strong. Inter J Anti Agen 2008;31:91-98.
  • Crooks SRH, Baxter AG, Traynor IM, Elliot C, McCaughey WJ. Detection of ivermectin residues in bovine liver using an enzyme immunoassay. Analyst 1998;123:355-358.
  • EMEA. Ivermectin (All mammalian food producing species). Committee for Medicinal Products for Veterinary Use. European Medicines Agency. http://www.ema.europa.eu/docs/en_GB/document_library/Maximum_Residue_Limits_-_Report/2014/05/WC500167329.pdf. Accessed May 15, 2018.
  • Wolstenholme AJ, Rogers AT. Glutamate-gated chloride channels and the mode of action of the avermectin/milbembycin anthelmintics. J Parasitol 2005;131:S85-S95.
  • Ộmura S, Crump A. Ivermectin: panacea for resource-poor communities? CellPress. Trends Parasitol 2014;30(9):445-455.
  • Escribano M, San Andrés MI, de Lucas JJ, Gonzáles-Canga A. Ivermectin residue depletion in Food producing species and its presence in animal foodstuffs with a view to human safety. Curr Pharm Biotecnhno l2012;13:987-998.
  • McKellar QA, Gokbulut C. Pharmacokinetic features of the antiparasitic macrocyclic lactones. Curr Pharm Biotechno 2012;13:888-911.
  • Lee Chiu S-H, Buhs R, Sestokas E, Taub R, Jacob T. Determination of ivermectin residue in animal tissues by High-Performance Liquid Chromatography-Reverse isotope dilution assay. J Agr Food Chem 1985;33:99-102.
  • Danaher M, Howells LC, Crooks SRH, Cerkvenik-Flajs V, O´Keeffe M. Review of methodology for the determination of macrocyclic lactone residues in biological matrices. J Chromato B 2006;844:175-203.
  • Shi W, He J, Jiang H, Hou X, Yang J, Shen J. Determination of multiresidue of avermectins in bovine liver by an indirect competitive ELISA. J Agr Food Chem 2006;54:6143-6146.
  • EuroProxima. Ivermectin ELISA, 5141IVER. http://europroxima.com/products/contaminants-and-residues/anthelmintics/ivermectin-elisa/; www.europroxima.com. Accessed May 15, 2016.
  • Lopez Panqueva RP. Useful algorithms for histopathological diagnosis of liver disease based on patterns of liver damage. Rev Colomb Gastroenterol 2016;31:443-457.
  • González Reina A, Palomares Velosa JE, Parra JL, Silva Sakzuk J, Abuabara Y, Mojica JE et al. Determination of organophosphorus, carbamate insecticides and ivermectin residues in raw milk from cattle farms in the Colombian low tropics. Abstract 10. Rev Colomb Cienc Pecu 2011;24:552-557.
  • Solis Rivera C, Wilcock A, Arellano Chavez S, Morales Loredo A, Mcewen S. Prevalence of ivermectin residues in cattle slaughtered in federally inspected abattoirs in Nuevo Leon, Mexico. Food Protect Trends 2011;31(4):212-215.
  • Rübensam G, Barreto F, Barcellos Hoff R, Mara Pizzolato T. Determination of avermectin and milbemycin residues in bovine muscle by liquid chromatography-tandem mass spectrometry and fluorescence detection using solvent extraction and low temperature cleanup. Food Control 2013;29:55-60.
  • Toutain PL, Upson DW, Terhune TN, McKensie ME. Comparative pharmacokinetics of doramectin and ivermectin in cattle. Vet Parasitol 1997;72:3-8.
  • Mugford CA, Kedderis GL. Sex-dependent metabolism of xenobiotics. Drug Meta Rev 1998;30(3):441-498.
  • Idowu ET, Alimba CG, Olowu EA, Otubanjo AO. Artenether-lumefantrine treatment combined with albendazole and ivermectin induced genotoxicity and hepatotoxicity through oxidative stress in Wistar rats. EJBAS 2015;2:110-119
  • Mohsen G, Pourjafar M, Badiei K, Habibi H. Ivermectin toxicity in a goat herd. Toxico Lett 2011;211S:43-216.
  • Veit O, Beck B, Steuerwald M, Hatz C. First case of ivermectin-induced severe hepatitis. Case report. Trans R Soc Trop Med Hyg 2006;100:795-797.
  • Yang CC. Acute human toxicity of macrocyclic lactones. Curr Pharm Biotechnol 2012;13:999-1003.
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