Inducción del metabolismo secundario de microorganismos mediante la estrategia OSMAC y la técnica de cocultivoaislamiento y elucidación estructural de los metabolitos secundarios producidos. Evaluación de su actividad biológica

Abstract

In the search for new bioactive natural products, in this study a selection of microorganisms (fungi and actinomycetes), mainly of marine origin, collected from different geographical regions, was investigated following an OSMAC (One Strain Many Compounds) approach for the induction of cryptic secondary metabolites, using different culture media, chemical elicitors and co-culture techniques with other microorganisms. This approach allowed the isolation of a wide variety of secondary metabolites, both new and known, which were subjected to different biological assays. The structures of the novel compounds were elucidated by HRESIMS and NMR, as well as by comparison with the literature, and QM-NMR (Quantum Mechanical - Nuclear Magnetic Resonance) computational methods were applied to determine their relative configurations. In some cases, absolute configurations were assigned by electronic circular dichroism or by comparison of the observed optical rotations with those of known structurally related natural products.