Tecnologías transcriptómicas y su potencial en la gestión del chancro resinoso del pino

  1. Zamora-Ballesteros, C. 1
  2. Martín-García, J 1
  3. Diez, J.J. 1
  1. 1 Sustainable Forest Management Research Institute, University of Valladolid—INIA
Journal:
Cuadernos de la Sociedad Española de Ciencias Forestales

ISSN: 1575-2410,  2386-8368

Year of publication: 2023

Issue: 49

Pages: 133-148

Type: Article

DIALNET GOOGLE SCHOLAR lock_openDialnet editor

More publications in: Cuadernos de la Sociedad Española de Ciencias Forestales

Abstract

Pine pitch canker (PPC) is a serious disease affecting Pinus spp. and Pseudotsuga menziesii worldwide. Several factors have contributed to the spread of the disease to all these continents, the most important being the international trade in plant reproductive material. Despite its importance, no effective measures are available to eradicate or control the disease either in nurseries or in the field. In this thesis, a compilation of current information on the pathways of spread of the pathogen was carried out, proposing preventive mechanisms to avoid its introduction into disease-free areas. We propose further research on the possible role of mycoviruses as biological control agents, exploring new methods of transfection with F. circinatum mycoviruses. We used transcriptomics technologies that, in addition to allowing us to study the effect of a mycovirus on its host fungus at the molecular level, provided useful information on genes, transcripts, and their functions associated with plant-pathogen interactions. Using RNA-Seq and susceptible and resistant species to F. circinatum, we identified genes and metabolic pathways associated with disease resistance traits and presented a comprehensive mapping of lncRNAs during pathogen infection. Our findings on the regulatory mechanisms involved in host defence will allow the optimisation of breeding programmes for the commercialisation of PPC-resistant pines.

Bibliographic References

  • De La Torre, A.R., Piot, A., Liu, B., Wilhite, B., Weiss, M., Porth, I., 2020. Functional and morphological evolution in gymnosperms: A portrait of implicated gene families. Evol. Appl. 13, 210–227. https://doi.org/10.1111/eva.12839
  • Dodds, P.N., Rathjen, J.P., 2010. Plant immunity: towards an integrated view of plant–pathogen interactions. Nature 11, 539–548. https://doi.org/10.1038/nrg2812
  • EFSA, 2010. Risk assessment of Gibberella circinata for the EU territory and identification and evaluation of risk management options. EFSA J. 8, 1620. https://doi.org/10.2903/j.efsa.2010.1620
  • EU Commission, 2019. The European Green Deal. European Commission, Brussels.
  • Gobierno de Navarra, 2018. Navarra sustituye el pino insignis por otras especies resistentes a los hongos. URL http://www.navarra.es/home_es/Actualidad/Sala+de+prensa/Noticias/2018/10/27/enfermedad+pino+insignis+hongos.htm (accessed 6.21.21).
  • Ioos, R., Aloi, F., Piškur, B., Guinet, C., Mullett, M., Berbegal, M., Bragança, H., Cacciola, S.O., Oskay, F., Cornejo, C., Adamson, K., Douanla-Meli, C., Ka?ergius, A., Martínez-Álvarez, P., Nowakowska, J.A., Luchi, N., Vettraino, A.M., Ahumada, R., Pasquali, M., Fourie, G., Kanetis, L., Alves, A., Ghelardini, L., Dvo?ák, M., Sanz-Ros, A., Diez, J.J., Baskarathevan, J., Aguayo, J., 2019. Transferability of PCR-based diagnostic protocols: An international collaborative case study assessing protocols targeting the quarantine pine pathogen Fusarium circinatum. Sci. Rep. 9, 8195. https://doi.org/10.1038/S41598-019-44672-8
  • Neale, D.B., Wegrzyn, J.L., Stevens, K.A., Zimin, A.V., Puiu, D., Crepeau, M.W., Cardeno, C., Koriabine, M., Holtz-Morris, A.E., Liechty, J.D., Martínez-García, P.J., Vasquez-Gross, H.A., Lin, B.Y., Zieve, J.J., Dougherty, W.M., Fuentes-Soriano, S., Wu, L.S., Gilbert, D., Marçais, G., Roberts, M., Holt, C., Yandell, M., Davis, J.M., Smith, K.E., Dean, J.F.D., Lorenz, W.W., Whetten, R.W., Sederoff, R., Wheeler, N., McGuire, P.E., Main, D., Loopstra, C.A., Mockaitis, K., deJong, P.J., Yorke, J.A., Salzberg, S.L., Langley, C.H., 2014. Decoding the massive genome of loblolly pine using haploid DNA and novel assembly strategies. Genome Biol. 15, 1–13. https://doi.org/10.1186/gb-2014-15-3-r59
  • Nystedt, B., Street, N.R., Wetterbom, A., Zuccolo, A., Lin, Y.-C., Scofield, D.G., Vezzi, F., Delhomme, N., Giacomello, S., Alexeyenko, A., Vicedomini, R., Sahlin, K., Sherwood, E., Elfstrand, M., Gramzow, L., Holmberg, K., Hallman, J., Keech, O., Klasson, L., Koriabine, M., Kucukoglu, M., Kaller, M., Luthman, J., Lysholm, F., Niittyla, T., Olson, A., Rilakovic, N., Ritland, C., Rossello, J.A., Sena, J., Svensson, T., Talavera-Lopez, C., Theiszen, G., Tuominen, H., Vanneste, K., Wu, Z.-Q., Zhang, B., Zerbe, P., Arvestad, L., Bhalerao, R., Bohlmann, J., Bousquet, J., Garcia Gil, R., Hvidsten, T.R., de Jong, P., MacKay, J., Morgante, M., Ritland, K., Sundberg, B., Lee Thompson, S., Van de Peer, Y., Andersson, B., Nilsson, O., Ingvarsson, P.K., Lundeberg, J., Jansson, S., 2013. The Norway spruce genome sequence and conifer genome evolution. Nature 497, 579–584. https://doi.org/10.1038/nature12211
  • PINESTRENGTH, 2015. Pine Pitch Canker - Strategies for management of Gibberella circinata in greenhouses and forests (COST Action FP1406: PINESTRENGTH).
  • Quan, M., Chen, J., Zhang, D., 2015. Exploring the secrets of long noncoding RNAs. Int. J. Mol. Sci. 16, 5467–5496. https://doi.org/10.3390/ijms16035467
  • Santini, A., Ghelardini, L., De Pace, C., Desprez-Loustau, M.L., Capretti, P., Chandelier, A., Cech, T., Chira, D., Diamandis, S., Gaitniekis, T., Hantula, J., Holdenrieder, O., Jankovsky, L., Jung, T., Jurc, D., Kirisits, T., Kunca, A., Lygis, V., Malecka, M., Marcais, B., Schmitz, S., Schumacher, J., Solheim, H., Solla, A., Szabò, I., Tsopelas, P., Vannini, A., Vettraino, A.M., Webber, J., Woodward, S., Stenlid, J., 2013. Biogeographical patterns and determinants of invasion by forest pathogens in Europe. New Phytol. 197, 238–250. https://doi.org/10.1111/j.1469-8137.2012.04364.x
  • Sturrock, R.N., Frankel, S.J., Brown, A.V., Hennon, P.E., Kliejunas, J.T., Lewis, K.J., Worrall, J.J., Woods, A.J., 2011. Climate change and forest diseases. Plant Pathol. 60, 133–149. https://doi.org/10.1111/J.1365-3059.2010.02406.X
  • Vainio, E.J., Bezos, D., Bragança, H., Cleary, M., Fourie, G., Georgieva, M., Ghelardini, L., Hannunen, S., Ioos, R., Martín-García, J., Martínez-álvarez, P., Mullett, M., Oszako, T., Papazova-Anakieva, I., Piškur, B., Romeralo, C., Sanz-Ros, A.V., Steenkamp, E.T., Tubby, K., Wingfield, M.J., Diez, J.J., 2019. Sampling and detection strategies for the pine pitch canker (PPC) disease pathogen Fusarium circinatum in Europe. Forests 10, 1–27. https://doi.org/10.3390/f10090723
  • Wegrzyn, J.L., Liechty, J.D., Stevens, K.A., Wu, L.S., Loopstra, C.A., Vasquez-Gross, H.A., Dougherty, W.M., Lin, B.Y., Zieve, J.J., Martínez-García, P.J., Holt, C., Yandell, M., Zimin, A.V., Yorke, J.A., Crepeau, M.W., Puiu, D., Salzberg, S.L., de Jong, P.J., Mockaitis, K., Main, D., Langley, C.H., Neale, D.B., 2014. Unique features of the loblolly pine (Pinus taeda L.) megagenome revealed through sequence annotation. Genetics 196, 891–909. https://doi.org/10.1534/genetics.113.159996
  • Wingfield, M.J., Brockerhoff, E.G., Wingfield, B.D., Slippers, B., 2015. Planted forest health: The need for a global strategy. Science 349, 832–836. https://doi.org/10.1126/science.aac6674
Data source: Dialnet