Differential distribution of acid-sensing ion channels and trp ion channels in peripheral sensory organs of adult zebrafish

  1. Cabo R. 1
  2. Viña E. 1
  3. Galvez A. 2
  4. Cobo T. 1
  5. Navarro M. 1
  6. Germanà A. 34
  7. Vega JA. 15
  1. 1 Departamento de Morfología y Biología Celular
  2. 2 Universidad de Castilla-La Mancha
    info

    Universidad de Castilla-La Mancha

    Ciudad Real, España

    ROR https://ror.org/05r78ng12

  3. 3 Dipartimento di Morfologia, Biochimica, Fisiologia e Produzione Animale, Sezione di Morfologia
  4. 4 University of Messina
    info

    University of Messina

    Mesina, Italia

    ROR https://ror.org/05ctdxz19

  5. 5 Universidad de Oviedo
    info

    Universidad de Oviedo

    Oviedo, España

    ROR https://ror.org/006gksa02

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Actas:
XXV Congress of the spanish Society of Anatomy

ISSN: 1136-4890,  2340-311X

Año de publicación: 2011

Volumen: 15

Número: S1

Páginas: 34

Congreso: XXV Congress of the spanish Society of Anatomy

Tipo: Aportación congreso

GOOGLE SCHOLAR lock_openAcceso abierto editor

Resumen

The peripheral sensory system of teleosts consists of spe-cialized sensory organs able to detect mechanical and chemi-cal environmental stimuli. The mechanosensory cells aregrouped into functional units called neuromasts that formthe lateral line system. The chemosensory cells are groupedinto taste buds and sparse in the lamellae of the olfactoryepithelium. Moreover scattered solitary chemosensory cellsare present in the skin. All these sensory cells contain ionchannels that are at the basis of the organ-specific transduc-tion that converts different types of stimuli into electricenergy. Acid-sensing ion channels (ASIC) monitore acid sen-sing and mechanoreception, while transient receptor (TRP)ion channels are involved in sensing temperature, light,pressure, as well as in detecting several chemical stimuli. Inthis study we used PCR, Westernblot and immunohistoche-mistry to detect ASIC and some TRP proteins in the peri-pheral sensory organs of adult zebrafish. In neuromasts weredetected TRPV4, ASIC2 and ASIC4; in the olfactory epi-thelim they were TRPV1, TRPV4, and all four ASIC pro-teins; finally, TRPV4, ASIC3 and ASIC4 were detected intraste buds. These results are of potential interest becausemutations in different TRP and ASIC channels have beenlinked to a variety of diseases, and zebrafish is a commonmodel used to investigate human diseases involving sensoryorgans.