Diffraction images of a crystal of the F-BAR domain of PSTPIP1 (Proline-serine-threonine phosphatase-interacting protein 1) mutant G258A (PDB entry 7AAL)

  1. Manso, Jose A 1
  2. Alcón, P 1
  3. Bayón, Y 2
  4. Alonso, A 2
  5. de Pereda, Jose M 1
  1. 1 Instituto de Biología Molecular y Celular del Cancer, Consejo Superior de Investigaciones Científicas, University of Salamanca, 37007 Salamanca, Spain
  2. 2 Instituto de Biología y Genética Molecular (IBGM), Consejo Superior de Investigaciones Científicas-Universidad de Valladolid, c/ Sanz y Forés 3, 47003 Valladolid, Spain

Verleger: Zenodo

Datum der Publikation: 2020

Art: Dataset

CC BY-SA 4.0

DOI: 10.5281/ZENODO.3874282 lock_openOpen Access editor

Zusammenfassung

Diffraction images of a crystal of the F-BAR domain of PSTPIP1 (residues 1-289), mutant G258A. Data were collected on a single crystal at the beamline i03 of the Diamond Light Source synchrotron (Didcot, UK) using radiation of 0.9999 Å wavelength and a PILATUS3 6M detector. The dataset consists of 2400 images (0.15 degree oscillation per image). Crystals belong to the space group P2(1)2(1)2(1) with unit cell dimensions a=48.19 Å, b=73.02 Å, c=205.25 Å. The asymmetric unit contains an homodimer of the F-BAR domain (~53% solvent content), which is the biological unit. Diffraction data was notably anisotropic. The lowest resolution limit was 2.92 Å in the direction b* and the highest limits were 1.97 Å and 2.09 in the directions a* and c*, respectively.   The structure derived form these data is published in: Manso, J.A., Marcos, T., Ruiz-Martín, V. Casas J, Alcón P, Sánchez Crespo M, Bayón Y, de Pereda JM, Alonso A PSTPIP1-LYP phosphatase interaction: structural basis and implications for autoinflammatory disorders. Cell. Mol. Life Sci. 79, 131 (2022). https://doi.org/10.1007/s00018-022-04173-w The structure is available at the PDB under the code 7AAL: https://www.ebi.ac.uk/pdbe/entry/pdb/7aal