Disposable amperometric biotool for peanut detection in processed foods by targeting a chloroplast DNA marker

  1. Gamella Carballo, María 1
  2. Ballesteros Redondo, Isabel 1
  3. Ruiz-Valdepeñas Montiel, Víctor 1
  4. Sanchiz Giraldo, África 1
  5. Cuadrado Hoyo, Carmen 2
  6. Pingarrón Carrazón, José Manuel 1
  7. Linacero de la Fuente, María Rosario 1
  8. Campuzano, Susana 1
  1. 1 Universidad Complutense de Madrid
    info

    Universidad Complutense de Madrid

    Madrid, España

    ROR 02p0gd045

  2. 2 Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España)
Journal:
Talanta

ISSN: 0039-9140

Year of publication: 2024

Volume: 277

Issue: 126350

Pages: 126350

Type: Article

DOI: 10.1016/J.TALANTA.2024.126350 GOOGLE SCHOLAR lock_openOpen access editor

More publications in: Talanta

Abstract

This work reports the development and application of a disposable amperometric sensor built on magnetic microcarriers coupled to an Express PCR strategy to amplify a specific DNA fragment of the chloroplast trnH-psbA. The procedure involves the selective capture of a 68-mer synthetic target DNA (or unmodified PCR products) through sandwich hybridization with RNA capture probe-modified streptavidin MBs and RNA signaling probes, labeled using antibodies specific to the heteroduplexes and secondary antibodies tagged with horseradish peroxidase. Amperometric measurements were performed on screen-printed electrodes using the H2O2/hydroquinone system. Achieving a LOD of 3 pM for the synthetic target, it was possible to detect 2.5 pg of peanut DNA and around 10 mg kg−1 of peanut in binary mixtures (defatted peanut flours prepared in spelt wheat). However, the detectability decreased between 10 and 1000 times in processed samples depending on the treatment. The Express PCR-bioplatform was applied to the detection of peanut traces in foodstuff.

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